Download High Content Screening: A Powerful Approach to Systems Cell by D. Lansing Taylor (auth.), D. Lansing Taylor, Jeffrey R. PDF
By D. Lansing Taylor (auth.), D. Lansing Taylor, Jeffrey R. Haskins, Kenneth A. Giuliano (eds.)
High content material screening (HCS) was once constructed via Cellomics Inc. within the mid-1990s to handle the necessity for a platform which may be utilized in the discovery-driven examine and improvement required to appreciate the services of genes and gene items on the point of the phone. excessive content material Screening: a strong method of structures cellphone Biology and Drug Discovery discusses its use as a excessive throughput platform to appreciate the features of genes, RNA, proteins, and different mobile ingredients on the point of the dwelling cell.
excessive content material Screening is assembled to help either current clients of HCS, in addition to investigators contemplating the addition of a discovery-driven platform to their study and improvement actions. The chapters were equipped into sections that spotlight the significance of integrating instrumentation, software software program, reagents, and informatics. moreover, there's a mix of natural overview chapters on key subject matters and particular equipment chapters.
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Additional resources for High Content Screening: A Powerful Approach to Systems Cell Biology and Drug Discovery
And Sedat, J. W. (1989) Solid-state imagers for microscopy, in 45. Fluorescence Microscopy of Living Cells in Culture, (Taylor, D. L. and Wang, Y. ), Academic, New York, pp. 291–313. 46 White, J. , Amos, W. , and Fordham, M. (1987) An evaluation of confocal versus conventional 46. imaging of biological structures by fluorescence light microscopy. J. Cell Biol. 105, 41–48. 47 Waggoner, A. (1990) Fluorescent probes for cytometry, in Flow Cytometry and Sorting, (Melamed, M. , 47. , and Mendelsohn, M.
2, in which an early biochemical screen revealed compounds with a range of activities from high micromolar to low nanomolar activity. In order to discriminate the potentially most efficacious of the low nanomolar compounds, we employed a HCS mitotic index assay in HeLa cells. The resulting separation of the cell-based “active” from the “inactive” compounds was quite striking, allowing the chemist to move the program successfully forward using a more finely tuned structure–activity relationship, one which accounted for the functional effects of the compound in a living cell.
Haskins, and K. , Totowa, NJ 19 20 Hoffman and Garippa are set up to acquire multiplexed HCS data on various biological events that can be made simultaneously by simply performing sequential reads of different emission spectra corresponding to the varying fluorophore probes of interest. One forward initiative is to use the complementary automation instruments that were initially installed in the biochemical HTS labs and to proceed with broad screening for cellular functions and phenotype elucidation.