Download E. Coli: Shiga Toxin Methods and Protocols (Methods in by Dana Philpott, Frank Ebel PDF

By Dana Philpott, Frank Ebel

A multidisciplinary panel of top experimentalists information the major protocols for the analysis and research of the pathogenesis of Shiga toxin-producing Escherichia coli (STEC) and its remoted cytotoxin (Stx). defined in step by step aspect, those without problems reproducible options variety from these for the analysis and detection of STEC micro organism in sufferer and animal samples, to these for learning the mobile microbiology of STEC infections, specifically host-pathogen interactions and the hemolysin of STEC. There also are protocols for learning the main points of Shiga toxin (Stx) biology-from the purification of the toxin to the consequences of Stx on a number of host mobilephone functions-and for exploring STEC-mediated illness in numerous animal types.

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Extra info for E. Coli: Shiga Toxin Methods and Protocols (Methods in Molecular Medicine)

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The silver stain-reactive component of the LPS is the polysaccharide portion (28). The many orderly spaced bands represent LPS molecules with varying numbers of repeating units in their O side chains (see Fig. 1). 9. The electrophoretic mobility of LPS in the SDS–polyacrylamide gel is determined by its lipid content and the number of repeating units (28,31). On 11% polyacrylamide gels, larger-size LPS bands migrate between 30 and 70 kDa and smaller bands between 10 and 15 kDa relative to protein molecular weight markers (see Figs.

11, 450–479. 6. Strockbine, N. , Wells, J. , Bopp, C. J. (1998) Overview of detection and subtyping methods, in Escherichia coli O157:H7 and Other Shiga Toxin-Producing E. coli Strains (Kaper, J. B. and O’Brien, A. D. ), American Society for Microbiology, Washington, DC, pp. 331–356. 7. , and Schmidt, H. (1999) Epidemiology and diagnosis of Shiga toxin-producing Escherichia coli infections. Diagn. Microbiol. Infect. Dis. 34, 229–243. 8. Karmali, M. , and Arbus, G. S. (1985) Sensitive method for detecting low numbers of verotoxin-producing Escherichia coli in mixed cultures by use of colony sweeps and polymyxin extraction of verotoxin.

Microbiol. 31, 1167–1172. 48. Chapman, P. A. and Siddons, C. A. (1996) A comparison of immunomagnetic separation and direct culture for the isolation of verocytotoxin-producing Escherichia coli O157 from cases of bloody diarrhoea, non-bloody diarrhoea and asymptomatic contacts. J. Med. Microbiol. 44, 267–271. 49. , and Datz, M. (1996) Isolation of enterohemorrhagic Escherichia coli O157 strains from patients with hemolytic– uremic syndrome by using immunomagnetic separation, DNA-based methods and direct culture.

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