Download E. coli. Shiga Toxin Methods and Protocols by Dana Philpott, Frank Ebel PDF

By Dana Philpott, Frank Ebel

A multidisciplinary panel of prime experimentalists information the major protocols for the analysis and learn of the pathogenesis of Shiga toxin-producing Escherichia coli (STEC) and its remoted cytotoxin (Stx). defined in step by step aspect, those with no trouble reproducible innovations diversity from these for the prognosis and detection of STEC micro organism in sufferer and animal samples, to these for learning the mobile microbiology of STEC infections, specially host-pathogen interactions and the hemolysin of STEC. There also are protocols for learning the main points of Shiga toxin (Stx) biology-from the purification of the toxin to the results of Stx on a variety of host telephone functions-and for exploring STEC-mediated ailment in a number of animal versions.

Show description

Read Online or Download E. coli. Shiga Toxin Methods and Protocols PDF

Best infectious diseases books

The Papillomaviruses

This quantity bargains an entire description of the present kingdom of biology about the papillomavirus. It evaluates the carcinogenic possibility to people posed via an infection with human papillomaviruses (HPVs). up to now, greater than 70 HPV forms were pointed out, of which over 15 were mentioned in cervical melanoma biopsies.

Molecular Virology

The e-book supplies a finished assessment at the wisdom of virus an infection proper for people and animals. for every virus relations the molecular info of the virus particle and the viral replication cycle are defined. when it comes to virus varieties with relevance for human and/or animal overall healthiness the knowledge on molecular biology, genetics and virus-cell interplay are mixed with these touching on, pathogenesis, epidemiology, clinics, prevention and remedy.

Mucosal Immunology

Mucosal Immunology, now in its fourth version, is the single entire reference protecting the fundamental technological know-how and medical manifestations of mucosal immunology. such a lot infectious brokers input the physique during the a number of mucous membranes, and plenty of universal infections ensue in or on mucous membranes, making this topic a space of singular significance within the box of immunology.

Handbook of Meningococcal Disease Management

This concise pocketbook will supply readers with an outline and heritage of meningococcal ailment, treatments and rising cures, and strategies of prevention. The ebook was once initially commissioned as a result of fresh advancements in vaccinations to avoid meningococcal illness. This ebook experiences the sickness development and linked chance elements; its pathogenesis and analysis; equipment of therapy and prevention, either present and rising; and the longer term instructions of meningococcal disorder administration.

Additional info for E. coli. Shiga Toxin Methods and Protocols

Example text

Acknowledgments The authors thank Olga Böhler and Dr. Kerstin Ludwig and Dr. Martina Bielaszewska for sharing protocols and technical advice. References 1. Karmali, M. A. (1989) Infection by verocytotoxin-producing Escherichia coli. Clin. Microbiol. Rev. 2, 15–38. 2. Paton, J. C. and Paton, A. W. (1998) Pathogenesis and diagnosis of Shiga toxinproducing Escherichia coli infections. Clin. Microbiol. Rev. 11, 450–479. 3. Karmali, M. , Fleming, P. , Arbus, G. , and Lior, H. (1985) The association between idiopathic hemolytic uremic syndrome and infection by verotoxin-producing Escherichia coli.

Dry overnight at room temperature. 26F 33 8/1/2002, 3:44 PM 34 Bitzan and Karch Fig. 1. E. coli O111:B4 lipopolysaccharide (Sigma), 6 µg/lane, fractionated by denaturing SDS-PAGE and silver stained, with protein molecular-weight markers of the indicated sizes. tion of 10 or 20 µg/mL PBS. Wash plates five times with 10 mM PBS containing 1% Tween. LPS-Coated microtiter plates can be stored at room temperature for several months. 2. 1% containing 2% FBS (v/v). Use triplicate wells. Incubate at 37°C overnight.

1965) Bacterial lipopolysaccharides: extraction with phenol–water and further applications of the procedure. Methods Carbohydr. Chem. 5, 83–91. 28. Tsai, C. M. and Frasch, C. E. (1982) A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal. Biochem. 119, 115–119. 29. , and Opferkuch, W. (1984) Analysis of electrophoretically heterogeneous lipopolysaccharides of Escherichia coli by immunoblotting. FEMS Microbiol. Lett. 22, 193–196. 30. Merril, C. , Sedman, S. , and Ebert, M.

Download PDF sample

Rated 4.41 of 5 – based on 13 votes