Download Biomembranes - Part D: Biological Oxidations by Sidney Fleischer, Lester Packer PDF

By Sidney Fleischer, Lester Packer

The severely acclaimed laboratory typical, Methods in Enzymology, is likely one of the such a lot hugely revered courses within the box of biochemistry. because 1955, each one quantity has been eagerly awaited, often consulted, and praised through researchers and reviewers alike. The sequence includes a lot fabric nonetheless correct this present day - actually an important e-book for researchers in all fields of existence sciences

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J. Steenkamp, B. A. C. Ackrell, T. P. Singer, and G. A. White, Arch. Biochem. Biophys. 178, 495 (1977). [6] RESOLUTION OF COMPLEX II 27 The specific activity of the enzyme is calculated using an extinction coefficient of Ered-ox 21 mM -1 cm -1 for DCIP at 600 nm. = Succinate Dehydrogenase Activity Measured by PMS-Mediated Reduction of DCIP. 0 ml. , 3'8°). A second cuvette containing water is placed in the reference compartment. Just before addition of enzyme, 16/xl of DCIP and 25/xl of PMS solution are mixed into the sample cuvette.

Hatefi, W. G. Hanstein, Y. Galante, and D. L. Stiggall, Fed. , Fed. A m . Soc. Exp. Biol. 34, 1699 (1975). 9 E. S. Baginski, P. P. , and B. Zak, Clin. Chem. 13, 326 (1967). ,0 L. Smith and H. Conrad, Arch. Biochem. Biophys. 63, 403 (1956). " K. Minnaert, Biochim. Biophys. Acta 50, 23 (1961). ,2 E. T. McGuinness and W. W. Wainio, J. Biol. Chem. 237, 3273 (1962). ':~T. Yonetani and G. S. Ray, J. Biol. Chem. 240, 3392 (1965). ,4 B. Errede, G. P. Haight, and M. D. Kamen, Proc. Natl. Acad. Sci. A. 73, 113 (1976).

Rosebrough, A. L. Farr. and R. J. RandalL, J. Biol. Chem. 193, 265 (1951). [7] P r e p a r a t i o n a n d P r o p e r t i e s o f Dihydroubiquinone : Cytochrome c Oxidoreductase (Complex III) By YOUSSEF HATEFI Dihydroubiquinone (QHz) + 2 ferricytochrome c ubiquinone (Q) + 2 ferrocytochrome c + 2 H + Several preparations of complex III (also referred to as cytochrome b c l ) have been reported. ~ and the subsequent method of Rieske 2 appear to result in prepa- rations of highest purity and enzymic activity.

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